For data in panels ( b‐d), p‐values were calculated using one‐way ANOVA and Dunnett's multiple comparisons post‐test. Data are the man + SEM of four independent experiments. LVS‐CM was heated to 65☌ for 30 min or 100☌ for 5 minutes and then added to PMNs. Data are the mean + SEM of six independent experiments. Effects of media that were conditioned by LVS for 4 or 24 hours. Data are the mean + SEM of three independent experiments. Medium was conditioned by LVS for 24 hours at 37☌, room temperature (RT), or 4☌ and then added to PMNs for 24 hours. Data are the mean ± SEM of three independent experiments. PMNs were infected with LVS at increasing MOI, and the ability of each iPMN‐CM to curtail apoptosis was tested. PMNs were left uninfected and untreated, were infected with LVS (iPMN), or were treated medium conditioned by infected PMNs (iPMN‐CM) or LVS (LVS‐CM), and apoptosis was quantified at 24 hours. The effects of MOI, time and temperature on apoptosis inhibition by conditioned media. p‐values were calculated using one‐way ANOVA and Dunnett's multiple comparisons post‐test. PMNs were left untreated or were infected LVS at the indicated time points. Delayed infection undermines apoptosis inhibition. Data are the mean + SEM of five independent experiments. Primary human monocyte‐derived macrophages were left untreated or were infected with wild‐type LVS or the Δ tolC mutant for 24 hours and cell death was measured by quantitation of lactate dehydrogenase release.
A tolC mutant is cytotoxic to human macrophages. Reminder: You can follow Pride Of Detroit on Twitter and like us on Facebook.Supplemental Figure 1. You can check out clips from Cleveland '95 on NFL.com and also find out when it will air again. CBS showed that picture during a preseason game last year, and it really never gets old. My personal favorite part of the documentary was getting to see the 1995 version of Schwartz again (as the top picture shows). He also talked about the experience of working as a "slappy" and how the impending move to Baltimore tore apart a team that was predicted by some to make the Super Bowl before the 1995 season. The funniest story Schwartz shared was one he's told before - about how he inadvertently ate Belichick's lunch one day. Schwartz appeared throughout the documentary to talk about his experiences working with Belichick. To say the least, there was a lot of talent working for the Browns when Belichick was the head coach. Also, Ozzie Newsome (current Ravens GM), Scott Pioli (current Chiefs GM), Mike Tannenbaum (current Jets GM), Thomas Dimitroff (current Falcons GM), George Kokinis (former Browns GM) and Michael Lombardi (former Raiders personnel executive) also worked for the Browns under Belichick. In addition to Schwartz, Nick Saban (current Alabama head coach), Kirk Ferentz (current Iowa head coach), Pat Hill (former Fresno State head coach) and Eric Mangini (former Jets and Browns head coach) worked under Belichick in Cleveland. What made the Belichick-led Browns so interesting, aside from the whole relocation controversy, was that so many people working under him went on to run their own team as a head coach or general manager. He officially was a college/pro scout, but really his job was to simply help out wherever he could - be it picking up somebody from the airport or buying cigarettes for the secretaries - while learning the trade under Bill Belichick. He was one of the "slappies," as they called them. This was the final team in Cleveland before the franchise relocated to Baltimore and became the Ravens, and the coaching staff and front office were filled with future stars in the world of football.ĭetroit Lions head coach Jim Schwartz was featured in the documentary since he worked for the Browns from 1993-95. Titled Cleveland '95, it focused on the 1995 Cleveland Browns, which were probably the most interesting 5-11 team in NFL history. Earlier this week, NFL Network premiered its latest A Football Life documentary.
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